Aspartic Proteinases: Retroviral and Cellular Enzymes

Voorkant
Michael N.G. James
Springer Science & Business Media, 31 mrt 1998 - 499 pagina's
The VIIth International Conference on Aspartic Proteinases was held in Banff, Alberta, Canada, from October 22 to 27, 1996. The venue was the Banff Centre in the Canadian Rockies, a setting well known worldwide for the scenic beauty and mountain grandeur. It was perhaps presumptuous of the organizers to call this the seventh Aspartic Proteinase Conference but it was felt that the meeting in 1982, organized by Tom Blundell and John Kay, was of an international stature and covered topics sufficiently broad to constitute a conference. Thus, there is a discontinuity in that the Gifu Conference organized by Prof. Kenji Takahashi was the fifth International Conference on Aspartic Proteinases. Officially, there has not been a sixth Conference and if there is confusion, it is the result of my desire to recognize the importance of the London meeting. Banffhosted 106 scientists from 14 different countries. There were 26 invited speak ers among the 44 oral presentations of the 7 main sessions. In addition, there were 53 con tributed poster presentations that spanned the whole range of interest in aspartic proteinases.
 

Inhoudsopgave

An Historical Overview
1
A Cellular AntiApoptosis Protein Is Cleaved by the HIV1 Protease
27
The Aspartic Proteinase from Equine Infectious Anaemia Virus
41
The Effect of Substrates on the Kinetics and the in Vivo Threshold Activity
47
A Comparison of gagpol Precursor Cleavage in Naturally Arising HIV Variants
53
Abstracts
71
Sensitivity to Inhibition and Catalytic Efficiency of HIV Proteinase Mutants
85
Computer Assisted Evaluation of Inhibitor Sensitivity to HIV1 PR Mutants
91
Crystal Structure of Human Pepsinogen A
259
Crystallographic Studies of an Activation Intermediate of Human Gastricsin
265
Abstracts
271
Crystal Structure of the Rhizomucor miehei Aspartic Proteinase
283
Rhizomucor miehei Aspartic Proteinases Having Improved Properties
293
Structure Biosynthesis and Specificity
315
Bacterial Aspartic Proteinases as Novel Antibiotic Targets
321
Structural Thermodynamic Study of the Binding of Renin Inhibitors
325

Investigation of an Allosteric Site of HIV1 Proteinase Involved in Inhibition
99
Mechanism of Action of Aspartic Proteases
115
Theory and Method of a Priori Computation of Catalytic Acts of Aspartic
123
Comparison of the Specificity of the Aspartic Proteinases Towards Internally
133
Expression of Chimeric Human Aspartic Proteinases
139
The Best Laid Plans of Mice and Men
147
Biosynthesis and Intracellular Targeting of the Lysosomal Aspartic Proteinase
153
An Aspartic Proteinase Expressed in the Equine Placenta
163
SiteDirected Mutagenesis
169
The Role
179
Epitope Mapping of Recombinant Human Procathepsin D
185
Construction of Chimeric Enzymes to Probe Subsite Contributions to Catalytic
191
Mass Spectrometry as a Tool for Studying the Action of Human Aspartic
201
Partial Characterization and Identification
207
Cathepsin E in the Central Nervous System
213
Detailed Analysis of Human Cathepsin E Prime Region Specificity
219
Proteolytic and NonProteolytic Activation of Prorenin
229
The Roles of the Basic Residues in the Prosegment of Aspergillopepsinogen I
239
Activation Mechanism of Pepsinogen as Compared to the Processing of
245
The Precursor Form of the Major Aspartic Proteinase from
253
Primary Substrate Specificities of Secreted Aspartic Proteases of Candida
335
Substrate Specificity of NonPepsinType Acid Proteinase Aspergillus niger
345
Overcoming the Unfavourable Entropic Contribution of Ligand Binding with
355
Abstracts
361
Screening Aspartyl Proteases with Combinatorial Libraries
375
Thermodynamics and Proton Uptake for Pepstatin Binding to Retroviral
381
Expression Purification and Characterization of the Recombinant Pepsin
387
Studies on Plasmepsins I and II from the Malarial Parasite Plasmodium
397
Plasmepsins I and II from the Malarial Parasite Plasmodium falciparum
407
Abstracts
413
Structural and Functional Aspects of Cardosins
423
Molecular Cloning of Aspartic Proteinases from Flowers of Cynara cardunculus
435
Aspartic Proteinases and Inhibitors in Plant Pathogenesis
441
Acid Proteinase from Nepenthes distillatoria Badura
453
A Comparative Study on the Aspartic Proteinases from Different Species
459
Purification
465
Abstracts
481
Author Index
489
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