In Vitro and in Vivo Analysis of the Role of PEX 19 P Farnesylation and PEX 14P Phosphorylation in Peroxisome BiogenesisLeuven University Press, 2006 - 88 pagina's |
Inhoudsopgave
LITERATURE OVERVIEW | 1 |
POSTTRANSLATIONAL MODIFICATIONS | 9 |
AIM AND OUTLINE OF THE STUDY | 19 |
CELL LINES AND STRAINS | 26 |
binding properties of Pex14p | 66 |
SUMMARY | 75 |
Veelvoorkomende woorden en zinsdelen
adrenoleukodystrophy amino acids antibodies Bgl II/Pst binding properties Biochem buffer C-terminal CAAX box casein kinase Cell Biol Chem CHO cells CK1 in vitro cytoplasm data not shown docking domain DsRed-PTS1 EGFP EGFP-F EGFP-Pex19p endoplasmic reticulum enzyme essential for peroxisome expressed farnesylation farnesylation motif farnesylation of Pex19p farnesyltransferase fibroblasts fraction Fransen FTase Fujiki function gene GST-HsPex19p hebben HsPex14p HsPex5p human Pex19p incubated inhibitor Invitrogen Kunau mammalian cells Mannaerts matrix protein import Matsuzono molecular mass markers mouse fibroblasts niet pastoris pathway peroxin peroxisomal matrix protein peroxisomal membrane proteins peroxisomal protein import peroxisomal targeting signal Pex14p-binding Pex19p variants Pex19p-deficient cells Pex19p-Pex14p interaction Pex2p Pex3p PEX5 Pex5p Pex6p Pex7p phosphorylation phosphorylation of Pex14p plasmid plasmid encoding PMPs posttranslational modifications prenylation PTS1 Rachubinski Rce1 receptor role Saccharomyces cerevisiae Sacksteder SDS-PAGE subcellular localization subcloned Subramani substrate transfected ubiquitin Vastiau Veldhoven vitro vitro binding vitro farnesylation vivo Western blotting wild-type yeast cells