Logic: Class in, 26;-Programme for 1886-87, 136;-Work Martin, H. N., 15;-Classes, 23, 67, 113, 122. Maryland, Correction of Statements relating to, in Memoirs o 34;-Archives of, edited by W. H. Browne, 42;-Great Mathematical Society, Proceedings, 38, 56, 64, 96, 116. Mathematics: Classes in, 22, 66;-Programme for 1886-87, 1885-86, 115;-American Journal of, 14, 51, 58, 72. Matriculates, 18. Matriculation Examinations, 100. Maurice, F., Letter from, 34. Maurice, F. D., Correction of Statements relating to Maryl of, 34. McMurrich, J. P., 15, 99;-Classes, 23, 67;-On the Existen Band of Cilia in Gasteropod Veligers, 5;-Notes on En Gasteropods, 85. Medical Science, On Some of the Humane Aspects of, W. Merriam, A. C., Law Code of the Kretan Gortyna, 47. Mills, T. W., Rhythm and Innervation of the Heart of the Modern Languages: Programmes for 1886-87, 125-128;-W Modern Language Notes, 47. Moore, J. L., Classes, 67, 68, 128. Morphology: Classes in, 23, 67;-Lectures on Selected P Naturalists' Field Club, 38, 56, 96, 122. Newcomb, Simon, 15;-Classes, 22, 66, 113, 115. Newell, H., 16, 114;-Classes, 26, 70. New Testament, Lectures on Printed, 29, 55;-Bibliograp Osborn, H. L., Metamorphosis of Limulus Polyphemus, 2. Osteology, Class in, 23, 67;-Programme for 1886-87, 121. Oysters, Artificial Propagation and Cultivation of, W. K. B Palmer, C. S., 51. Partitions, Note on, D. Barcroft, 64. Pathology: Lectures on, 32;-Programme for 1886-87, 123. Peabody Institute Lectures, 28. Pedagogics: Classes in, 26, 70;-Programme for 1886-87, 1885-86, 136. Peirce, C. S. and J. Jastrow, Small Differences of Sensation Perkins, C. A., 15, 114;-Classes, 22, 66, 117, 118. Philology, American Journal of, 14;-see Ancient and Mod Philosophy, History of: Classes in, 26, 70;-Programme fo Work of 1885-86, 136. Photograph of the Normal Solar Spectrum, 39, 41, 95. Physical Geography: Classes in, 70;-Programme for 1886-8 of 1885-86, 135. Physical Laboratory, Laying the Corner-stone of, 99;-Pla Physical Laboratory in Modern Education, H. A. Rowland, Physical Training: Lectures on, 31;-Bibliography to lectu Physics: Classes in, 22, 66;-Programme for 1886-87, 1 1885-86, 118. Physics at the Johns Hopkins University, 52. 885-86, 136. D. Maurice, in Memoirs Welch, 101. ks, 86. a-Turtle, 6; The Cardiac -isons, 8. -86, 120;- of 1885-86, ems in, 99; 65;-Reso- to Lectures, ks, 10. NIVERSITY CIRCULARS. Physiology: Classes in, 23, 67;-Programme for 1886-87, 121;-Work of Physiology of the Heart of the Alligator, T. W. Mills, 7. Politics, History of: Classes in, 25, 70;-see Historical and Political Science. Proceedings of Societies, 38, 56, 64, 96. Psychology: Classes in, 26, 70;—Programme for 1886-87, 135;—Work of Psycho-Physics: Classes in, 26, 70. Publications issued under Auspices of the University, 14, 58, 73, 95, 140. Putnam, F. W., Methods of Archæological Research, 64, 89. Records of Virginia Company, J. F. Jameson, 61. Register of Officers and Students, 15–21;—Additions to, 33, 51. Reinach, S., Inscribed base of an archaic Bronze Statue from Mt. Ptous, 47; Renouf, É., 16, 51;-Classes, 22, 66, 114, 119. Review of Ten Years' Work of the University, J. C. Thomas, 107. Rhythm and Innervation of the Heart of the Sea-Turtle, T. W. Mills, 6. Roman Law, Programme, 133. Rome, Archæological Discoveries in, A. L. Frothingham, 64. Rowland, H. A., 15;-Classes, 22, 66, 113, 117, 118;-Photograph of Solar Sanskrit and Comparative Grammar of Cognate Languages: Classes in, 24, Shemitic Languages: Classes in, 24, 68;-Programme for 1886-87, 125;— Sibylline Books and Teaching of the Apostles, J. Rendel Harris (notice), 44. Skin, Motor Sensations of, G. S. Hall and H. H. Donaldson, 36. Smyth, H. W., Classes, 23, 67, 128;-Reduction of ε to i in Homer, 35;— Societies, see University Societies. Solar Spectrum, Photograph of, 39, 41, 95. Spanish Class in, 25, 69;-see Modern Languages. Special Students, Roll of, 20. Speech Mixture in French Canada, A. M. Elliott, 62. Spieker, E. H., 15;—Classes, 23, 67, 68, 114, 124, 128, 133;-Genitive Abso- iii Sternberg, G. M., Malarial "Germ" of Laveran, 95. Story, W. E., 15;-Classes, 22, 66, 114, 115, 117. Strassburg, Earliest Municipal Code of, J. M. Vincent, 63. Students, Roll of, 1885-86, 16-20, 33, 51;-Roll, 1876-86, 74-79. Students' "House of Commons," Constitution and By-Laws of, 48;-Dis- cussion of the Constitution of, 49. Studies from the Biological Laboratory, 14, 123. Studies in Historical and Political Science, 14, 40, 43. Sturgis, Russell, Greek Sculpture in the Museums of Athens, 64. Sylvester, J. J., 15. Syriac Manuscript with the Antilegomena Epistles, Reproduction of, 41. Teaching of the Apostles and the Sibylline Books (notice), J. R. Harris, 44. Ten Years' Work of Johns Hopkins University, Review of, J. C. Thomas, 107. Tenth Anniversary of Johns Hopkins University, 100;-Addresses and Thomas, J. C., Review of Ten Years' Work of the University, 107. Thomson, Sir William, Letter, 52. Thucydides, Krüger's Chronology of the so-called "Summary" of, C. D. Todd, H. A., 15;-Classes, 25, 69, 114, 127, 131. Undergraduates, Roll of, 18, 19;-Examinations of, 100. University Examinations of Undergraduates, 100. University Scholars for 1885-86, 20, 33. University Societies: Communications to, 34, 59, 93–95;-Proceedings, 38, Veda, Correlation of v and m in, M. Bloomfield, 93. Vincent, J. M., On the Manuscripts of Prof. J. C. Bluntschli, 59;-Earliest Virginia Company, Records of, J. F. Jameson, (notice), 61. Ward, W. H., Recent Discoveries in Assyria and Babylonia, 54, 89. Warren, M., 15;-Classes, 23, 67, 114, 124, 128, 133. Washington Scholarships, 53. Wedmore, Frederick, Lectures, 28. Welch, W. H., 15-Lectures on Pathology, 32;-Lectures on Micro- Whitney, W. D., Sis and Sa Aorists (6th and 7th Aorist-forms) in San- Williams, G. H., 15;—Classes, 23, 67, 114, 119, 120;-Lectures on Inorganic Williams, Langdon, Letter, 49. Williams Manuscript, Reproduction of, in Photograph, 41, 95. Wilson, H. V., 99. Wood, H., 15, 51;—Classes, 24, 68, 69, 114, 125, 126, 130. Woodworth, C. L., 16, 114;—Classes, 26, 70. Wright, J. H., Programme, 113, 124. Yager, A., Finanzpolitik im Nordamerikanischen Bürgerkrieg (notice), 64. 121. PUBLICATIONS OF THE JOHNS HOPKINS UNIVERSITY. I. American Journal of Mathematics. S. NEWCOMB, Editor, and T. CRAIG, Associ- II. American Chemical Journal. I. REMSEN, Editor. Bi-monthly. 8vo. Volume III. American Journal of Philology. B. L. GILDERSLEEVE, Editor. Quarterly. 8vo. IV. Studies from the Biological Laboratory. (Including the Chesapeake Zoölogi- The Johns Hopkins University Circulars are printed by Messrs. JOHN MURPHY & CO., No. 182 West Baltimore Street, Baltimore, Baltimore Street, Baltimore. CHESAPEAKE ZOOLOGICAL LABORATORY, EIGHTH SESSION. Metamorphosis of Limulus Polyphemus. By H. L. Osborn, Abstract of Researches on the Embryology of Limulus polyphemus. By W. K. Brooks and A. T. Bruce, On the Chemical Composition, etc. of the Blood of Limulus polyphemus, etc. By W. H. Howell, The Presence of Haemoglobin in the Echinoderms. By W. H. Howell, On the Existence of a Post-oral Band of Cilia in Gasteropod Veligers. By J. P. McMurrich, The Rhythm and Innervation of the Heart of the Sea Turtle. By T. Wesley Mills, On the Physiology of the Heart of the Alligator. By T. Wesley Mills, The Cardiac Rhythm of Fishes, and the Action of Drugs, etc. By T. Wesley Mills, Origin of the Endoderm in Lepidoptera. By A. T. Bruce, On the Artificial Propagation and Cultivation of Oysters in Floats. By W. K. Brooks, Notes on the Stomatopoda. By W. K. Brooks, - Note on the Fishes of Beaufort Harbor, N. C. By O. P. Jenkins, CHESAPEAKE ZOOLOGICAL LABORATORY MARINE STATION OF THE JOHNS HOPKINS UNIVERSITY The marine laboratory of the university was opened at Beaufort, N. C., on May 23, and it was occupied for research until September 15. As the party was much too large for the house which was used in past years, a second one, which was vacant for the summer, was rented and occupied, but even with this additional space, several applicants were refused, from lack of room. The following is a list of the members of our party: W. K. BROOKS, Ph. D., Associate Professor of Morphology, J. H. U.-Director. E. A. Andrews, Ph. B., Fellow, J. H. U. A. T. Bruce, A. B., Fellow by Courtesy, J. H. U. G. B. Haldeman, A. B., Graduate Studen', J. H. U. J. C. Hemmeter, M. D., Graduate Student, J. H. U. F. H. Herrick, A. B., Graduate Student, J. H. U. W. H. Howell, Ph. D., Associate, J. H. U. O. P. Jenkins, A. B., Terre Haute, Ind. T. W. Mills, M. D., Lecturer on Physiology, McGill University, Montreal. J. P. McMurrich, Ph. D., Instructor, J. H. U. A. D. Morrell, Chapel Hill, O. H. F. Nachtrieb, S. B., Prof. of Zoology, University of Minnesota. The following abstracts indicate the scope and character of the work which was carried on. The Metamorphosis of Limulus polyphemus. By Prof. HENRY L. OSBORN, Purdue University, La Fayette, Indiana. Explanatory note by W. K. Brooks. Prof. Osborn has kindly placed at my disposal the notes and drawings which he made at the marine laboratory at Beaufort in 1884 from the eggs of Limulus which he had procured from the oviducts and had fertilized artificially. He made a very careful study of the superficial changes in the egg, and the young larva, and cut sections through the eggs at a very early stage of their development, but as he did not study the later stages in the same way he failed to understand his sections, and I have, therefore, made a few slight changes in his notes, in order to bring them into harmony with the conclusions which have been reached by Mr. Bruce and myself in our study of the same subject this summer. W. K. BROOKS. Soon after fertilization the yolk contracts and shrinks away from the chorion, and a number of deep irregular fissures make their appearance running in towards the centre and cutting up the yolk into masses which resemble cleavage segments. The fissures are very irregular and present a great variety of forms. Some of the eggs remain almost perfectly smooth and round, while others become deeply furrowed, and nearly all of them pass through the furrowed stage, although I did not observe any in which there was a perfectly regular segmentation of the surface. The irregularity of these changes would seem to indicate that they are pathological, although they do not prevent the eggs from undergoing normal development, to its completion. In from one to three days after fertilization the egg exhibits a structure which is the first indication of normal development. In surface view this structure resembles a circular pit or depression with a dark centre, surrounded by a circle of lighter color. My sections at this stage show that it is not a depression, but a protoplasmic cap, situated upon the surface of the yolk. It stains deeply; but is perfectly clear and transparent, and I could find in it no nucleus or trace of a cellular structure. It soon disappears and its relation to the future parts of the embryo was not determined. The time at which this structure appears is very inconstant and there is also the greatest difference in the rate of development among the individual eggs, and some are much more tardy in arriving at the various stages and emerge from the shell a day or two later than the most rapidly developing ones. During the night of the 26th (the eggs were fertilized July 23d) the pit disappeared, and at 8 a. m. on the 27th a white mound was observed upon the surface, at once recalling the primitive cumulus of Claparède, and Balfour. It is very constantly present, but I do not know its relation to the future parts or to the pit which preceded it. On July 28th, 11.30 a. m., a deep semicircular depression showed itself. On the 29th, in the space between the two lips of the depression of the day before, a pair of buds appears-the beginnings of the anterior pair of limbs. On the following day two more pairs are added, and in front of the first pair and behind the front lip of the fold, a most important structure is for the first time seen, it is a slit elongated antero-posteriorly; the definitive mouth opening. It is distinctly in front of the first pair of limbs. It is to be noted that the anal opening has not yet shown itself according to my observations. The stomodeum and the three somites are now included in an area which is plainly marked off from the rest of the egg and surrounded by an oval elevation. On the following day, July 31, there had appeared inside this rim the remaining pairs of cephalo thoracic appendages. On the 31st a thickening along the middle ventral line marked out the nervous system. This lot of eggs was followed and the embryos all hatched in due course, though the circumstances in which they were placed were very unfavorable. Twenty healthy eggs were carried in a 4 oz. bottle inland and development went on normally, and on August 16, 24 days after fertilization, the first one of the lot hatched and the others all followed it, straggling along for several days. Others left at Beaufort hatched in due time. Observations of the surface from the time that the limbs are marked off do not add anything to the facts described by Packard and Dohrn, except that the statement of Packard that the compound eyes appear upon the third segment is wrong. They appear very plainly upon the fourth somite as stated by Dohrn. Abstract of Researches on the Embryology of Limulus polyphemus. By W. K. BROOKS and A. T. BRUCE. Segmentation. At the end of the process of segmentation, sections show that the entire yolk of the large and totally opaque egg consists of an uniform mass of large spherical yolk cells, each with its nucleus. They are of uniform size from the centre to the surface, and are closely packed with yolk globules and granules, but they are not flattened by mutual pressure, since their outlines are nearly circular. The changes which the egg undergoes in reaching this stage are very peculiar, but its total opacity prevents superficial examination, and as there are no external marks by which a series of eggs can be cut in the same place, it is difficult to form a complete picture of the course of segmentation, although the process appears to be as follows: In a ripe, unfertilized egg the yolk consists of a homogeneous mass of yolk globules and granules, covered at one point by a superficial aggregation or cap of protoplasm. In sections of an egg twenty-four hours after fertilization, protoplasmic processes or pseudopodia extend from the cap into the yolk, and surrounding and including the substance of the yolk, divide this up into a number of yolk-balls, each of which consists of an investing sheath of protoplasm with an inclosed sphere of yolk, but they contain no nuclei, nor were any found at this stage in the protoplasmic cap. On the third and fourth days after fertilization the yolk-balls are smaller and more numerous, with sharper outlines, and one or two large, sharplydefined oval nuclei were visible in the protoplasmic cap. On the fifth day this begins to decrease in size, and ultimately disappears, while the yolk-balls become more numerous, and a very small nucleus becomes visible in each of them. The yolk is so opaque and so packed with granules that we were unable to decide whether the nuclei are on the surface or in the interior of the yolk-balls, and we learned nothing regarding the origin of the nuclei. The Origin of the Blastoderm. After segmentation is completed the cells nearest the surface become smaller than the central ones, probably as the result of cell division. These peripheral cells are at first rounded and filled with yolk, like the central ones, but they soon become transparent, flatten |