Ion Channels, Volume 207Bernardo Rudy, Linda E. Iverson Gulf Professional Publishing, 18 sep 1997 - 917 pagina's This book describes modern electrophysiological techniques for recording and analysis of ion channels in various systems including cells in culture, Xenopus oocytes, brain slices, cellular organelles, and microorganisms. Many chapters deal with the prevention and correction of electrophysiological artifacts. Also included are methods for the purification and expression of ion channel genes and proteins. Key Features * Applications of electrophysiological techniques * Expression in heterologous systems * Reconstitution of lipid bilayers * Purification and characterization of ion channel proteins and genes * Recording of ion channels of intracellular membranes and microorganisms * Data storage and analysis |
Inhoudsopgave
ERWIN NEHER | 3 |
KORENBROT 28 Department of ida 33101 | 5 |
Constructing a Patch Clamp Setup RICHARD A LEVIS | 14 |
Probing Molecular Structure and Structural M COLOMBINI S PENG | 29 |
Glass Technology for Patch Clamp Electrodes JAMES L RAE | 66 |
Ion Channel Selectivity Permeation and Block TED BEGENISICH | 92 |
Access Resistance and Space Clamp Problems As CLAY M ARMSTRONG | 100 |
Correction for Liquid Junction Potentials in Patch ERWIN NEHER | 123 |
Bilayer BARBARA E EHRLICH | 463 |
Surface Charge Effects on Ion Conduction in Ion RAMÓN LATORRE | 471 |
ANA MARIA Garcia 33 Eisai Research | 491 |
Determination of Ion Permeability by Fluore ANA MARIA GARCIA | 501 |
Synthetic Peptides and Proteins as Models for ANNE GROVE TAKEO IWAMOTO | 510 |
Purification and Reconstitution of Skeletal Muscle VINCENT FLORIO | 529 |
Purification and Reconstitution of Nicotinic Ace AMITABH CHAK | 546 |
Purification Affinity Labeling and Reconstitution HUBERT REHM | 556 |
Nonstationary Noise Analysis and Application to STEFAN H HEINEMANN | 131 |
Prevention of Rundown in Electrophysiological RICHARD HORN | 149 |
Patch Voltage Clamping with LowResistance WILLIAM M ROBERTS | 155 |
Perfusing Patch Pipettes J M TANG J WANG | 176 |
Recording from CalciumActivated Potassium B S PALLOTta A L Blatz | 194 |
PatchClamping Cells in Sliced Tissue Prepara FRANCES A EDWARDS | 208 |
From Gene to HERMONA SOREQ | 225 |
SHLOMO SEIDMAN 14 Department of Bio fornia Medical School and Division | 265 |
Maintenance of Xenopus laevis and Oocyte Injec ALAN L GOLDIN | 266 |
TANG 1048 Department of Physiol Molecular Physiology Yale University | 271 |
JENWEI LIN 42 Department of Physiology B S Pallotta 12 Department of Phar | 278 |
Preparation of RNA for Injection into Xenopus ALAN L GOLDIN | 279 |
Tissue RNA as Source of Ion Channels and Recep TERRY P SNUTCH | 297 |
In Vitro Synthesis of RNA for Expression of Ion RICHARD SWANSON | 310 |
Electrophysiological Recording from Xenopus WALTER STÜHMER | 319 |
Use of Stage IIIII Xenopus Oocytes to Study DOUGLAS S Krafte | 339 |
Intracellular Perfusion of Xenopus Oocytes NATHAN DASCAL | 345 |
Recording of Gating Currents from Xenopus STEFAN H HEINEMANN | 353 |
LigandBinding Assays in Xenopus oocytes AMY L BULLER | 368 |
Expression of Gap Junctional Proteins in Xenopus Lisa Ebihara | 376 |
Stable Expression of Heterologous Multisubunit TONI CLAUDIO | 391 |
Vaccinia Virus as Vector to Express Ion Channel ANDREAS KARSCHIN | 408 |
Expression of Ion Channels in Cultured Cells ALEXander Kamb | 423 |
FRECH 40 Department of PETER L JØRGENSEN 38 The August | 424 |
Insertion of Ion Channels into Planar Lipid Bi PEDRO LABARCA | 447 |
Affinity Purification and Reconstitution of Cal DAN A KLÆRKE | 564 |
Assay and Purification of Neuronal Receptors for SUNIL R HINGORANI | 573 |
Isolation of Ion Channel Genes by Expression GEORGES C FRECH | 592 |
Hybrid Arrest Technique to Test for Functional ILANA LOTAN | 605 |
Cloning of Ion Channel Gene Families Using the ELEAZAR VEGASaenz | 613 |
Overview of Toxins and Drugs as Tools to Study TOSHIO NARAHASHI | 620 |
Overview of Toxins and Drugs as Tools to Study TOSHIO NARAHASHI | 643 |
Biosynthesis of Ion Channels in CellFree and WILLIAM B THORNHILL | 659 |
Patch Clamp Techniques to Study Ion Channels BERNHARD U Keller | 673 |
Patch Clamp Studies of Microbial Ion Channels YOSHIRO SAIMI BORIS MARTINAC | 681 |
Patch J M TANG J WANG | 692 |
Planar Bilayer Recording of Ryanodine Receptors ROBERTO CORONADO | 699 |
Software for Acquisition and Analysis of Ion ROBERT J FRENCH | 711 |
Stationary SingleChannel Analysis MEYER B JACKSON | 729 |
Analysis of Nonstationary SingleChannel Currents F J SIGWORTH AND J ZHOU | 746 |
Preventing Artifacts and Reducing Errors in Sin K L MAGLEBY | 763 |
Analysis of Drug Action at SingleChannel Level EDWARD MOCZYDLOWSKI | 791 |
Analysis of Sodium Channel Tail Currents GABRIEL COTA | 806 |
ARMSTRONG 555 Department of of Maryland College Park Maryland | 807 |
Calculation of Ion Currents from Energy Profiles OSVALDO ALVAREZ | 816 |
WILLIAM A CATTERALL 35 Department California 94305 | 835 |
AUTHOR INDEX | 855 |
887 | |
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Veelvoorkomende woorden en zinsdelen
acid activity added addition allow analysis applied assay bandwidth bilayer binding block brain buffer Ca2+ calcium capacitance cells centrifuge charge cloned components concentration conductance constant containing cytoplasm dependence described determined dish effect electrical electrode estimate example experiments expression filter frequency function gating gene given glass headstage important increase incubation injection ion channels isolated lipid London mean measured medium membrane method mRNA Nature noise obtained oocytes patch clamp performed perfusion Physiol pipette possible potential preparation present Proc procedure produce properties protein pulses receptors recording reduce removed resistance response seal selectivity series resistance shown shows single single-channel solution specific stage step subunits surface techniques temperature tion tissue transcription transient tube types vesicles voltage clamp volume whole-cell Xenopus
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